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Rexgenero-France (previously aratinga.bio), a preclinical stage biotech company developing an off-the-shelf and universal chimeric antigen receptor (CAR)-T therapy which allows in vivo targeting and transduction of T-cells, today announces the presentation of a novel approach for quantitative real-time particle analysis of lentivirus vectors at the Antibody Industrial Symposium 2020 on Monday 23 November 2020 at 18:00 CET.

The study was conducted in collaboration with Myriade to explore new processes to measure lentiviral vector physical titre for: (1) characterization of lentiviral products for use in CAR-T cell products following purification and concentration; and (2) in-process controls during upstream and downstream processing.

Using Myriade’s Videodrop (Interferometry Light Microscopy), a new type of equipment that measures the concentration of nanoparticles from a single drop of sample, the research team achieved results consistent with the standard lentiviral vector physical titration methods (ELISA p24 protein quantification and Tunable Resistive Pulse Sensing).

“Accurate measurements of lentiviral vector physical titres are crucial, as the titre largely determines transduction efficiency and the number of integrated gene copies in the target cell. Developing reliable and efficient methods for measuring lentiviral vector physical titres is integral to production of our CAR-T cell components and the development of rigorous in-process controls,” commented, Dr. Cecile Bauche, Vice President and Chief Scientific Officer at Rexgenero. “We are excited to present data collected using Myriade’s Videodrop at AIS 2020. Compared to the standard lentiviral vector physical titration processes, this novel approach is real-time, label-free and filtration-free, and could overcome challenges faced using the traditional methods. We look forward to further work with the Myriade team.”

Details of the poster presentation are as follows:

• Event: Antibody Industrial Symposium 2020
• Title: Novel approach for quantitative real-time particle analysis of lentiviral vectors
• Date: 23 November 2020
• Time: 18:00 PM-19:00 PM (CET)
• Session: Poster Session
• Presenters: Cécile Bauche (Rexgenero France) / Marie Berger & Matthieu Greffet (Myriade)
• Conference link: https://aiscongress.com/

Videodrop films in real time all particles between 10 nm and 10 μm in a drop. No labelling is needed, and the process is real-time, label-free, filtration-free, non-destructive and only requires 5–10 μL sample volume.